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Figure 5 | BMC Microbiology

Figure 5

From: Rapid identification of Brucella isolates to the species level by real time PCR based single nucleotide polymorphism (SNP) analysis

Figure 5

Alignments of various 16SrRNA sequences around the location of the 16S rRNA 1055 probes. Alignments of fragments of 16S rRNA centred around SNP at base 1055 relative to B. abortus 9–941 showing location of the SNP, used in combination with the 16S rRNA771/778 SNPs to separate Brucella from closely related bacteria. The alignment contains 65 sequences taken directly from Genbank and includes many examples of Ochrobactrum, the closest phylogentic neighbour of Brucella as well as other, less-closely related, members of the α-proteobacteria. In this figure, the targetted Brucella specific SNP is highlighted in blue and the non-Brucella alternatives in red. The green bar above both figures represents the location of probe hybridisation. The alignment includes only one Brucella sequence as there is 100% identity in the 16SrRNA sequences between all Brucella [34]. Whilst this assay on its own is not as discriminatory as 16S rRNA771/778 assay, it crucially distinguishes non-Brucella not detected by the afore mentioned assay.

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