Figure 1

Schematic vector maps of SvH3P.pac and SvH3G.pac. A) Schematic plasmid map of the SvH3P.pac shuttle vector that includes an S. vortens H3 promoter for transcription of eGFP (enhanced GFP), an AU1 epitope tag, and a tandem affinity purification (TAP) tag for affinity purification (GAT = GFP+AU1+TAP). This plasmid also includes a puromycin resistance gene (pac) for selection in S. vortens flanked by the α-tubulin 5'UTR and 3'UTR regions (SvATB5p and SvATB3p), and an ampicillin resistance gene (amp) for antibiotic selection in E. coli. B) Schematic plasmid map of the SvH3G.pac shuttle vector. In this plasmid, the S. vortens histone H3 gene (including ~100 bp of the H3 histone native promoter (SvH3p) was fused to and includes a 12 amino acid hydrophobic linker, as well as other selectable markers (see Figure 1A). Relevant restriction sites are also highlighted in the vector maps, as well as M13R and T7 sequencing primer regions.