Figure 8

Lrp protein levels as a function of growth. Wild-type strains of E. coli, P. mirabilis and V. cholerae were grown in MOPS glucose plus nicotinate or MOPS glucose defined-rich media. The data are from two independent experiments (open and closed symbols). Growth curves (A-C, MOPS glucose medium; G-I, MOPS rich medium) and Lrp protein levels (D-F, glucose; J-L, rich) are shown. Equal amounts of total protein were loaded in each lane, and a standard curve of purified E. coli Lrp was included on each gel for quantitation. M shows a comparative western blot. Cell pellets were boiled and equal amounts of total protein from E. coli (Ec) and P. mirabilis (Pm) were resolved side-by-side via SDS polyacrylamide gel electrophoresis. The subsequent blot was probed with polyclonal antiserum raised against E. coli Lrp.