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Table 4 Oligonucleotide primers

From: Multi locus sequence typing of Chlamydiales: clonal groupings within the obligate intracellular bacteria Chlamydia trachomatis

Target gene

Forward primer

Reversed primer

gatA

GCTTTAGAATTARSARAWGCT

GATCCTCCGGTATCYGATCC

oppA_3

ATGCGCAAGATATCAGTGGG

AAAGCTCCRSTWGMTATMGGWAG

hflX

GCTTCTARAGTACTTTTAAATG

TATTTRGAAATYTTTKCSAGYCG

gidA

GGAGTCWCTACWAAAGAAGG

TCGTAYTGYACATCRAAAGG

enoA

CCTATGATGAATCTKATCAATGG

TCTTCTTCRGCWAGMCCATCT

hemN

AGATCTTCTTCWGGRGGWAGAGA

TTCYTTCAKAACSTAGGTTTT

fumC

ATTAAAAAATGTGCTGCT

CCTTCAGGAACATTYAACCC

  1. R = A or G; S = G or C; W = A or T; Y = C or T; M = A or C; K = G or T;
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BMC Microbiology

ISSN: 1471-2180

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