Skip to main content
Figure 2 | BMC Microbiology

Figure 2

From: The presence of the pilus locus is a clonal property among pneumococcal invasive isolates

Figure 2

The genetic structure of the region downstream of the pfl gene where the rlrA islet is found among piliated isolates. Panel A. Agarose gel of the PCR products resulting from the amplification of the region downstream of the pfl gene using primers PFL-up and P-dn. m – DNA ladder (1 kb plus, Invitrogen, Carlsbab, CA). Lane 1 – isolate presenting a region type C. Lane 2 – isolate presenting a region type A. Lane 3 – isolate presenting a region type B. Lane 4 – strain R6. Sizes of the fragments in kilobases are indicated in the left. Panel B. Structure of the region found downstream of the pfl gene in four isolates and designated region type B compared to the rlrA islet and flanking regions of strain TIGR4. Genes are indicated by arrows and primers by vertical lines. Genes encoding proteins with similar functions are represented by the same pattern. The fragments with high DNA similarity between the regions compared are represented by black and grey boxes connected by lines (analysis was performed using the GATA software). Panel C. Structure of the region found downstream of the pfl gene in 68 isolates and designated region type C compared to the corresponding region of strain R6 (region type A).

Back to article page