Table 4 Oligonucleotide primers and probes used for RT-PCR
From: AI-2-dependent gene regulation in Staphylococcus epidermidis
Gene product | Â | Oligonucleotide(5'-3') |
|---|---|---|
Acetoin dehydrogenase | Forward | GAGCAAGCTAAAGAGGCTGGTTAT |
| Â | Probe | CCTTGAAATGCTGATTGAGTCACTTTCACAT |
| Â | Reverse | CCTTTGATTAACGCCTTTGCAT |
Gluconokinase | Forward | AGTAAAACCAGGTGCAGAAGGATT |
| Â | Probe | CGCGCTCTCCAGCTAAATAGGG |
| Â | Reverse | CGTCAGCGTTCCACAATGG |
Antiholin-like protein LrgB | Forward | TGTCGCGGCAGCTAAAGAA |
| Â | Probe | ACTGCAATACTTCCCATTGATTCTTCAG |
| Â | Reverse | CAACAATAACGCCAACGATGAC |
Nitrite extrusion protein | Forward | GGTTGGTGGTGTTATAGGTGATAAATTT |
| Â | Probe | AAGCGCTTATCATCGACTTCGTG |
| Â | Reverse | GCTTAATATAAGCGCACCAATAATCAT |
PTS system, fructose-specific IIABC components | Forward | TTAATCACAGCAAACAACTCTCACAT |
| Â | Probe | CAATGAGTGATTTATCGAACACCATAT |
| Â | Reverse | TCTTGTCATTGGTTCCTTGGTAATT |