Table 3 Nucleotide sequences of primers for genes whose expression in both 400 μm vs. 100 μm and 200 μm vs. 100 μm biofilms was compared
From: DNA-microarrays identification of Streptococcus mutans genes associated with biofilm thickness
ORFa | Primer Sequences (5' – 3') | |
|---|---|---|
| Â | Forward | Reverse |
16S rRNA | CCTACGGGAGGCAGCAGTAG | CAACAGAGCTTTACGATCCGAAA |
SMU.125 | TGGCACATGCACGAGAAGAA | GGCCCGGAATAGCATAGTTG |
SMU.442 | CTTTCAGGCGGGATGTTGAG | GCTGTCTGGCGGTTTCAATC |
SMU.574c | TGGTCATACAGTTGTGCAGC | GACGAGGCCGATGCAACA |
SMU.609 | CAGTTGTGAACGTGGCTGAAA | TGAGCTGCTGCCTTATCTGAAA |
SMU.618 | GCACTTATCGCTTGCGGTTT | CACCTGACAATACCAGCAACCA |
SMU.744 | TGTTCAGGTTGCGTCAACCTT | AATGACACGGCGAAGAGCTT |
SMU.987 | GCACGCTTGCAGTACATTGC | CATAAGGTCGCGAGCAGCT |
SMU.1488c | ACGCCATCTCATCAGCCTTT | ACCACTTACCCAATCGCTTGA |
SMU.1889c | CTGCTGTATCTGCCCCTGCT | CCTAAATCCCAACCAATTGCTG |
SMU.2146c | CAGGAGCTTCAGGTCTCTTTCAAA | CCTTGGGCTTTATAAGCATTGATAG |