Table 1 Primers and amplification conditions for the different PCR based screening of genes that encode for antibiotics

PCA (C, D)

PCA2a

PCA3b

5'-TGCCAAGCCTCGCT CCAAC-3'

5'-CGCGTTGTTCCTCGTTCAT-3'

77

Initial denaturation 94°C for 3 min, 30 cycles of 94°C for 60 s, 58°C for 45 s, and 72°C for 60 s. Final extension at 72°C for 10 min.

PCA (X, Y)

30–84/1

30–84/2

5'-CAGTTCATCCGGCGGGCTGCAG-3'

5'-CCCGTTTCAGTAAGTCTTCCATG-

ATGCG-3'

77

Initial denaturation 94°C for 3 min, 30 cycles of 94°C for 60 s, 58°C for 45 s, and 72°C for 60 s, Final extension at 72°C for 10 min.

PCN

PhzH-up

PhzH-low

5'-CGCACGGATCCTTTCAGAATGT-

TC-3'

5'-GCCACGCCAAGCTTCACGCTCA-3'

78

Initial denaturation 94°C for 30 s, 30 cycles of 94°C for 30 s, 64°C for 30 s, and 72°C for 7 min. Final extension at 72°C for 10 min.

DAPG

Phl2a

Phl2b

5'-GAGGACGTCGAAGACCACCA-3'

5'-ACC GCAGCATCGTGTATGAG-3'

79

Initial denaturation 94°C for 90 s, 35 cycles of 94°C for 35 s, 53°C for 30 s, and 72°C for 45 s. Final extension at 72°C for 10 min.

PLT

PltBf

PltBr

5'-CGGAGCATGGACCCCCAGC-3'

5'-GTGCCCGATATTGGTCTTGACCG-

AG-3'

79

Initial denaturation 94°C for 2 min, 29 cycles of 94°C for 1 min, 58°C for 45 s, and 72°C for 1 min, Final extension at 72°C for 10 min.

PRN

Prncf

Prncr

5'-CCACAA GCCCGGCCAGGAGC-3'

5'-GAGAAGAGCGGGTCGATGAAG-

CC-3'

79

Initial denaturation 94°C for 2 min, 30 cycles of 94°C for 1 min, 58°C for 45 s, and 72°C for 1 min. Final extension at72°C for 10 min.