Time-dependent C4 deposition on C. parapsilosis and reference strains C. albicans , C. neoformans , S. aureus and E. coli in MBL-deficient serum supplemented with exogenous purified human MBL. Microorganisms were incubated with 10% MBL-deficient serum with or without 2.5 μg/mL purified human MBL, at 37°C over a time course of 10 min. Results are expressed as median fluorescence (MFI). Data are the mean ± SEM of 3 separate experiments. *P < 0.05 and ** P< 0.005 unpaired Student's t test of MFI.