Ca2+-dependent interaction between mannose-binding lectin (MBL) and C. parapsilosis and reference strains C. albicans , C. neoformans , S. aureus and E. coli. Microorganisms were incubated in the presence of 5 μg/mL purified human MBL in VSB2+. After incubation with mouse anti-MBL monoclonal antibody and FITC-labeled goat anti mouse IgG, binding of MBL to these microorganisms was analyzed by flow cytometry. Results are expressed as median fluorescence intensity (MFI). Data are the mean ± SEM of 3 separate experiments. Solid bars, addition of human purified MBL; open bars, no addition of human purified MBL.