Table 2 Primer combinations used to validate each fimbrial mutation.
Primer combination | Predicted amplicon size (bp) | Sequence (5'-3') |
|---|---|---|
bcfAFOR + C1 | 633 | TGCACTATCCGCAACGATATATTT |
bcfAREV + C2 | 507 | TAAAATACGCTTTCGCGATCGGTCGGT |
csgAFOR + C2 | 173 | CAAGGAGCAATAAAGTATGCATAATTT |
csgAREV + C1 | 302 | CAGCAGTTGTAGTGCAGAAACAGTCGCATA |
lpfAFOR + C2 | 867 | TTAGTTACGCGCTGTGTCAA |
lpfAREV + C1 | 288 | ATCCAATACCCACCTCTATACACTCCA |
fimAFOR +C1 | 807 | AACCTCAGATCGCACCTGCTGC |
fimAREV + C2 | 429 | ATGCCGACATGACGCCAGACC |
sefAFOR + C1 | 373 | CTATTAATGGGGATGTTGTGTAA |
sefAREV + C2 | 946 | CTAATAATCTCTTATAATTTC |
safAFOR + C1 | 701 | TGAGACTCTCTCATTGGAGCGCT |
safAREV + C2 | 597 | AATTGAGGTCAAGGGTCGCGCC |
stbAFOR + C2 | 887 | TTAATGGTGGGGGACATCGTA |
stbAREV + C1 | 295 | TTATTTTTACCACTCCATAAGCACGAA |
pegAFOR + C2 | 179 | CACAAGCCAGGCATAATGCAATCATC |
pegAREV + C1 | 377 | ACATTGCGATAACTTCCTGTCTATGAGAA |
stdAFOR + C2 | 587 | GCTGTACCGTACCTGACTGTC |
stdAREV + C1 | 714 | TGTTTTTAAATTTCATCCGCGAAG |
steAFOR + C1 | 739 | TACGACAACGCCTATATAATA |
steAREV + C2 | 600 | AGCAGCGTGGAGTGTCCCAGGTCAGC |
stfAFOR + C1 | 283 | CATATAAACATGGGGTATTGATGA |
stfAREV + C2 | 155 | GGCTGGCATCATCTTTAACA |
sthAFOR + C1 | 584 | GCGTTGATTTTGTTAATGC |
sthAREV + C2 | 704 | GAAAGCTCACGATTTGAGATCAAC |
stiAFOR + C2 | 385 | TTTGGCCGACAACACACTATG |
stiAREV + C1 | 661 | GTAAATCAGCTTAAATTCCG |
C1 | - | TTATACGCAAGGCGACAAGG |
C2 | - | GATCTTCCGTCACAGGTAGG |