Functional identification of HugZ, a heme oxygenase from Helicobacter pylori

BMC Microbiology

Table 1 Oligonucleotide primers used in this study

Primer	Sequence(5' → 3')	Characteristics	Functions (genes)
H1	CGCGCATATGCTTAATCGTATC	NdeI	To amplify gene hugZ
H2	GGCCTCGAGTTTCTTGTGAGCG	XhoI
LA-F1	CGAGCTCCCATTACTACTGCTACTACTA	SacI	To amplify left arm of gene hugZ (LA)
LA-R1	GCTCTAGAGCACCGCTCATAAGGGGCAA	XbaI
RA-F1	TCCCCCGGGGGAAATATTCTCCTTAGTT	SmaI	To amplify right arm of gene hugZ (RA)
RA-R1	ACGTCGACGCAATGCTTTTAGAAATTAA	SalI
H-F	TCGCTAAACAAACAGAATCAA	/	To detect hugZ
H-R	ATGTGTTCTATGATACGATTAAGCAT	/
P-F	GCTCTAGAGCTTGCCCCTTATGAGCGGT	/	To detect hugZ or cam
P-R	CTTATTTTTTGAAACTAAGGAGAATATT	/
hugZ-1	TTGGGCAAGTCCATCACG	245 bp	RT-PCR/Real-time RT-PCR Evaluation of hugZ
hugZ-2	GGTCGCTAAACAAACAGAA
gyrB-1	CGCTAAAGAAAGTGGCACGA	267 bp	RT-PCR/Real-time RT-PCR Evaluation of gyrB (normalizer)
gyrB-2	TGCGCGTTTCTTCATCCAT

The underlined sequences are the restriction sites. /: Absence of restriction endoenzyme sites.

ISSN: 1471-2180