Figure 3

Oxidative, heat and salt stress sensitivities at different cell densities. Strains used are wild-type R. etli CNPAF512 (circles); rel _Ret mutant CMPG8705 (squares) and the complemented strain CMPG8705/pCMPG8715 (triangles) grown in TY medium. Results are presented as percentages, calculated as the number of CFUs in the stressed sample relative to the number of CFUs of the corresponding control sample. Control samples were incubated at 30°C for the same time period as treated samples. (A) Samples were incubated for 30 min in the presence of 10 mM H₂O₂ in a 30°C water bath. The experiment was repeated 3 times and a representative graph is shown. (B) Samples were incubated for 60 min in a 45°C water bath. The experiment was repeated 2 times and a representative graph is shown. (C) Samples were incubated for 6 hours in a 30°C shaking incubator in the presence of a final concentration of 2.5 M NaCl.