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Table 3 Immunoblot results of the Ct recombinant proteins related those of the vector tags.

From: Evaluation of an in silico predicted specific and immunogenic antigen from the OmcB protein for the serodiagnosis of Chlamydia trachomatis infections

Vector

 

Tag-Ct reactivity/Tag reactivity

PinPoint Xa-1

No. of sera

BPT-Ct+/BPT+

BPT-Ct +/BPT-

BPT-Ct -/BPT+

BPT-Ct -/BPT-

  

12

0

0

6

pGEX-6P-1

No. of sera

GST-Ct+/GST+

GST-Ct+/GST-

GST-Ct-/GST+

GST-Ct-/GST-

  

2

2

0

14

  1. BPT: biotin purification tag expressed using the native PinPoint Xa-1 vector, BPT-Ct: the C-terminal (Ct) recombinant protein expressed using the PinPoint Xa-1 vector, GST: glutathione-S-transferase expressed using the native pGEX-6P-1 vector, GST-Ct: the C-terminal (Ct) recombinant protein expressed using the pGEX-6P-1 vector.
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BMC Microbiology

ISSN: 1471-2180

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