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Figure 2 | BMC Microbiology

Figure 2

From: Uptake and intra-inclusion accumulation of exogenous immunoglobulin by Chlamydia-infected cells

Figure 2

Bovine IgG is sequestered within the chlamydial inclusion. Merged images from a cross-sectional Z-series of a representative J774A.1 macrophage infected with C. trachomatis shows IgG distributed throughout the entire inclusion. Starting from the medial portion of the cell and sectioning downwards in 1 μm increments, co-localization (yellow) of chlamydial antigens and bovine IgG is observed throughout the thickness of the inclusion, indicating that IgG is contained within this compartment. For orientation, an "N" demarcates the nucleus that has been cross sectioned in the lowermost slice. Chlamydial organisms were labeled with a polyclonal rabbit anti-Chlamydia antibody and detected with TRITC-conjugated goat anti-rabbit IgG. IgG was labeled using a FITC-conjugated F(ab')2 goat anti-bovine IgG. (bar = 10 μm, section thickness = 0.5 μm)

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