Figure 1
From: Functional characterization of a small heat shock protein from Mycobacterium leprae
Comparison of the localization and solubilisation in urea of sHsp18 protein (A) and Mce* protein (B). To check whether the sHsp18 protein forms inclusion bodies on over expression, the protein profile of the soluble and insoluble protein fractions were checked on a 12% SDS-PAGE. Lanes contain the soluble fraction (lane 1), the insoluble fraction (lane 2) of the crude sonication extract, and the supernatants after incubation of the insoluble fraction with suspension buffer containing 2 M urea (lane 3), 4 M urea (lane 4), 6 M urea (lane 5), and 8 M urea (lane 6). C&D. 2D gel analysis to show the presence of sHsp18 in the outer membrane fractions of E. coli. 45 μg protein was loaded on a 7 cm IPG strip with a pI range covering 4–7. After the second dimension, the gel was stained with colloidal coomassie. sHsp18 and OmpA (marker for outer membrane fraction) have been shown. 'C' represents outer membrane fraction and 'D' represents inner membrane fraction.