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Table 3 Effects of recombinant protein on spleen cell proliferation in mice injected with recombinant proteins and a control group of mice injected with PBS by MTTa

From: The expression and antigenicity of a truncated spike-nucleocapsid fusion protein of severe acute respiratory syndrome-associated coronavirus

Group

Proliferation (D valueb ± SD) with the following concentration (μg/ml) of recombinant proteins

 

10

3

1

0.3

0

PBS Control

0.116 ± 0.112

0.074 ± 0.046

0.011 ± 0.002

0.022 ± 0.008

0.043 ± 0.008

truncated S-N

0.603 ± 0.118

0.551 ± 0.019

0.542 ± 0.216

0.476 ± 0.274

0.068 ± 0.021

  1. a Four BALB/c mice of each group were killed. Spleens were harvested and lymphocyte cultures (Three repeats) were stimulated in vitro for 3 days with medium or with various concentrations of purified recombinant fusion truncated S-N protein (three repeats). The truncated S-N protein could induce T cell proliferation and significant differences were found between the groups of mice immunized with recombinant protein or injected with PBS (T test P = 0.0084 < 0.05).
  2. b D Value = D570-D630-blank