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Figure 3 | BMC Microbiology

Figure 3

From: Proinflammatory effect in whole blood by free soluble bacterial components released from planktonic and biofilm cells

Figure 3

Induction of cytokine gene transcription in human whole blood by free-soluble material released by live A. actinomycetemcomitans cells. qRT-PCR was used to quantify the levels of IL-1β, IL-6, IL-8, MIP-1β, and TNF-α mRNA in human whole blood after stimulation with planktonic A. actinomycetemcomitans strain D7S for 6 h. The stimulation was done in the presence (+PMB) and in the absence (-PMB) of polymyxin B. Shown is the increase (fold change) of each cytokine mRNA in whole blood stimulated with strain D7S relative to no bacteria controls (50% serum in PBS) from two separate experiments (P < 0.05).

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