Figure 2

Western and Northern analyses of PstS expression. A) Western blot to detect cell-bound PstS in S. lividans TK24 grown under the indicated conditions (B, basal; G, basal + 5% glucose, F, basal + 5% fructose) and times (48, 60 hours). 20 μg of total protein were loaded per lane. B) Northern analysis of pstS expression in the above cultures. 16S RNA was used as a loading control (lower part). C) Transcriptional analysis of the pstS operon genes in the presence of 5% fructose at the indicated times (48, 60 hours). The bands detected are indicated at the right. 16S RNA was used as a loading control (lower part). D) Schematic representation of the pst operon and surrounding genes. A putative transcriptional terminator is proposed between pstS and pstC. The sizes of the RNA bands obtained in the Northern are indicated.