Figure 1From: Expression of the pstS gene of Streptomyces lividansis regulated by the carbon source and is partially independent of the PhoP regulatorExtracellular PstS does not participate in phosphate incorporation. A) Western blot to detect extracellular (S) and cell-bound (C) PstS in the indicated S. lividans strains. (20 μg of total protein were loaded per lane). B) Uptake of 32P-labeled phosphate after 1 hour at 30°C. Strains assayed: wild-type S. lividans (wt); the ΔpstS deletion mutant (ΔpstS); the complemented transformant ΔpstS (ΔpstS+pINTUF5), and the same mutant containing the integrative fusion xylanase signal peptide-PstS (ΔpstS+pINTUF9). The results presented are the means of three independent experiments.Back to article page