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Figure 1 | BMC Microbiology

Figure 1

From: Expression of the pstS gene of Streptomyces lividansis regulated by the carbon source and is partially independent of the PhoP regulator

Figure 1

Extracellular PstS does not participate in phosphate incorporation. A) Western blot to detect extracellular (S) and cell-bound (C) PstS in the indicated S. lividans strains. (20 μg of total protein were loaded per lane). B) Uptake of 32P-labeled phosphate after 1 hour at 30°C. Strains assayed: wild-type S. lividans (wt); the ΔpstS deletion mutant (ΔpstS); the complemented transformant ΔpstS (ΔpstS+pINTUF5), and the same mutant containing the integrative fusion xylanase signal peptide-PstS (ΔpstS+pINTUF9). The results presented are the means of three independent experiments.

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