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Figure 8 | BMC Microbiology

Figure 8

From: Structural Prediction and Mutational Analysis of the Gifsy-1Xis Protein

Figure 8

Limited proteolysis of wild-type Gifsy-1 Xis and Gifsy-1 Xis substitution mutants S51P and C17Y. Lanes 1, 3 and 5 contain protein without the addition of proteinase K. Lanes 2, 4, and 6 contain the indicated Gifsy-1 wild-type protein or substitution mutants S51P or C17Y. Limited proteolysis was accomplished using a protein to proteinase molar ratio of 80:1. Peaks corresponding to wild-type Gifsy-1 mass spectroscopy results are indicated.

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