Table 1 The sequences of DNA sequencing primers
From: Expression of recombinant Clostridium difficile toxin A and B in Bacillus megaterium
| Â | Â | tcdA Sequencing Primers |
|---|---|---|
| Â | Â | Sequence |
TcdA-Seq1 | Forward | CTGCAGCATCTGACATAG |
TcdA-Seq2 | Forward | AAGTTATGAAGCAACATGC |
TcdA-Seq3 | Forward | TCATCTCCATCTATAAGTTCTC |
TcdA-Seq4 | Forward | GTTTCTGGAAATTGTTTGG |
TcdA-Seq5 | Forward | GTTACTGGATGGCAAACC |
TcdA-Seq6 | Reverse | TAGTCCAATAGAGCTAGGTC |
TcdA-Seq7 | Reverse | CCATGTCCAATAAAGGTTAC |
TcdA-Seq8 | Reverse | ACTGCTCCAGTTTCCCAC |
TcdA-Seq9 | Reverse | ACATTTCTACCATTTCCG |
TcdA-Seq10 | Reverse | ATAACCAGTTGAGGCTATG |
| Â | Â | tcdB Sequencing Primers |
| Â | Â | Sequence |
TcdB-Seq1 | Forward | GAACAAGAGTTGGTAGAAAG |
TcdB-Seq2 | Forward | TCTTGGTGAAGATGATAATC |
TcdB-Seq3 | Reverse | CCTGGTAACATATCAACATC |
TcdB-Seq4 | Reverse | CTCTCTCTGAACTTCTTGC |
TcdB-Seq5 | Forward | CCTACATTATCTGAAGGATTAC |
TcdB-Seq6 | Forward | GATGTTGATAATGTTGTGAGAG |
TcdB-Seq7 | Forward | ATAGTAAGCCTTCATTTGG |
TcdB-Seq8 | Reverse | GCTGCACCTAAACTTACAC |
TcdB-Seq9 | Reverse | ATTACTTCCATTTACCTCAC |
TcdB-Seq10 | Forward | TTATAGAGGAGCTGTAGAATG |
TcdB-Seq11 | Reverse | GCTTTACCTGTTTCTGGG |
| Â | Â | Sequencing Primers in pHis-1522 vector |
| Â | Â | Sequence |
phis-seq-F | Forward | TTTGTTTATCCACCGAACTAAG |
phis-seq-R | Reverse | TGATTGGCTCCAATTCTTG |