Figure 4
From: Demonstration of IS711 transposition in Brucella ovis and Brucella pinnipedialis
Southern blot hybridisation of amplified regions from TcR mutants showing the insertion of IS 711. PCR products obtained with primers G11 and G12 from several pGBG1/TcR insertion mutants were digested with HaeIII and hybridised with an IS711 probe. Lane 1: IS711 probe, lanes 2–10: insertion mutants containing IS711, lane 11: PCR product from pGBG1. Molecular sizes of the fragments are indicated in bp.