Figure 4

Quantification of PXO99 _GFP expressing GFP using a fluoremeter or ELISA. A. Fluorescence of the bacteria recovered from the rice leaves was directly measured with a fluoremeter (excitation, 480 nm; emission, 520 nm). The bars indicate average of the fluorescence of each sample and the error bars are standard deviations from six technical replicates of two biological replicates. B. GFP protein expressed in PXO99_GFP under host condition were quantified using ELISA, Inoculated bacterial strains, PXO99 (circle) and PXO99_GFP (triangle), were recovered from the scored rice leaves of TP309 (close) and TP309-XA21 (open) at 0, 4, 8, and 12 DAI. ELISA using anti-GFP polyclonal IgG antibody then carried out. Each value represents averages of the absorbance at 480 nm and standard deviation from three leaves per treatment. Inset is the bacterial growth curve with linear scale. This experiment was repeated three times with more than ten rice leaves.