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Table 2 Sensitivity of the LAMP assay for V. parahaemolyticus

From: Development of a loop-mediated Isothermal amplification assay for sensitive and rapid detection of Vibrio parahaemolyticus

Strain

Samples

 

Dilutions of cultures for the assays

   

10-1

10-2

10-3

10-4

10-5

V. parahaemolyticus

       

AQ4980

       
 

Pure cultures

CFU per reaction

196.3

19.6

2.0

0.2

0.02

  

LAMP

+

+

+

± (1/3)

-

  

PCR

+

+

± (1/3)

-

-

 

Spiked shrimps

CFU per reaction

196.3

19.6

2.0

0.2

0.02

  

LAMP

+

+

+

-

-

  

CFU per reaction

19.6

2.0

0.2

0.02

0.002

  

PCR

+

-

-

-

ND

  1. +, triplicate assay showed all positive.
  2. ±, triplicate assay showed both positive and negative (positive number/tested number).
  3. -, triplicate assay showed all negative.
  4. ND, Not determined.