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Table 1 Bacterial strains, plasmids and primers used in this study.

From: Development of a luciferase-based reporter system to monitor Bifidobacterium breve UCC2003 persistence in mice

Bacterial strains

Relevant properties

Source/Reference

Escherichia coli TOP10

F-mcr A φ80lac Z_M15 lac X74rec A1ara D gal U rps L end A1 nup G

Invitrogen, UK.

Bifidobacterium breve UCC2003

Electroporation host

UCC Culture Collection.

Plasmids

Relevant properties

Source/Reference

pPl2lux

Allows for translational fusions to lux ABCDE operon

[2]

pUC19

2.686 kb vector based on pMB1, Ampr

Fermentas

pUC19-lux

pUC19 containing the 5.6 kb modified lux ABCDE operon as SalI – PstI fragment.

[2]

pBC1.2

Bifidobacterial shuttle vector based on pBC1, Cmr

[14]

pLuxMC1

pBC1.2 containing the modified lux ABCDE operon

This study

pLuxMC2

pBC1.2 containing the lux ABCDE operon plus promoter of repC from pBC1

This study

pLuxMC3

pBC1.2 plus lux ABCDE operon with P help [3].

This study

Primers

Sequence

Source/Reference

IM111

Aaaaggacgatttcggttgg

[2]

IM112

Ccaatgccccagaaatttcc

[2]

P rep F

Ccatccaactcgag gcacaagccgcgcgagcggtc

This study

P rep R

Catgggcactagtgtacgtc

This study

  1. Ampr, ampicillin resistance; Cmr chloramphenicol resistance. Underlining indicates restriction sites used in subsequent cloning steps