Figure 4

Immunoblot analysis of mAb binding to rALO and B. anthracis Sterne strain. rALO, culture supernatant, and lysed bacterial cells were solubilized in Laemmli sample buffer, run under denaturing conditions and were separated on a 10% SDS-PAGE. The blots were incubated with rALO specific mAbs and isotype specific GAM secondary Ab conjugated to HRP. MW, molecular weight marker; +, rALO; P, lysed bacterial cells; S, culture supernatant. The expected molecular weight of rALO is approximately 53 Kd.