Evaluation of an immunomagnetic separation method to capture Candida yeasts cells in blood

BMC Microbiology

Table 1 Analytical sensitivity of Candida cells capture with IMS method

Strains	Time (hours) of positive blood culture (mean ± SD)^a	CFU/ml in initial contaminated blood	CFU/ml after IMS (mean ± SD)^a	Immunocaptured cells (% ± SD)^ab
Candida albicans 5575	25.45 ± 0.59	32	8.8 ± 0.6	27.5 ± 1.9
Candida albicans ATCC 66396	25.95 ± 1.46	6	1.75 ± 0.3	29.1 ± 3.8
Candida krusei 5374	22.26 ± 0.48	30	3.25 ± 0.5	10.8 ± 1.7
Candida krusei 5481	16.20 ± 0.28	40	17.3 ± 2.5	43.2 ± 6.2
Candida glabrata 5511	25.05 ± 0.5	20	7.6 ± 1.3	38 ± 6.4
Candida glabrata 5484	25.38 ± 0.58	10	3.8 ± 0.6	38 ± 6.3
Candida tropicalis 5437	19.02 ± 0.88	30	4.8 ± 2.2	16 ± 3.8
Candida tropicalis 5395	17.51 ± 0.50	30	4.3 ± 2.2	14.3 ± 7.3

^acalculation of mean and SD is derived from three different experiments
^bpercentage of immunocaptured cells: number of CFU per 1 ml of blood after IMS procedure/number of CFU per 1 ml of blood in initial contaminated blood
CFU: Colony Forming Unit, IMS : ImmunoMagnetic Separation, SD : Standard Deviation

ISSN: 1471-2180