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Table 3 Oligonucleotide primers and probes used in real-time multiplex PCR assay.

From: Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples

Target gene

Primer or Probe

Sequence (5'-3')

Tm (°C)

Amplicon Size (bp)

Reference or Accession No.

sefA

Forward

GTGGTTCAGGCAGCAGTTACT

61.1

334

L11008

 

Reverse

CAGGGACATTTAGCGTTTCTTGAG

59.7

  
 

Probe

ROX-CAGCTCAGAATACAACATCAGCCAACTGG-BBQ

66.2

  

fliC

Forward

CCCCGCTTACAGGTGGACTAC

60.2

433

AY649721

 

Reverse

AGCGGGTTTTCGGTGGTTGT

63.6

  
 

Probe

YAK-TAAAGCCGCATTGACAGCAGCAGGTG-BBQ

69.8

  

aceK

Forward

CCGCGCTGGTTGAGTGG

62.0

240

U43344

 

Reverse

GCGGGGCGAATTTGTCTTTA

60.3

  
 

Probe

FAM-AACCACTGCCGAACTGTATATGGCGA-BBQ

65.0

  

sdf

Forward

AAATGTGTTTTATCTGATGCAAGAGG

57.6

299

[35]

 

Reverse

GTTCGTTCTTCTGGTACTTACGATGAC

58.7

  
 

Probe

Cy5-CGAATGGTGAGCAGACAACAGGCTGATTTA-BBQ

68.4

 

This study