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Table 1 Bacteria and plasmids

From: Hfq regulates the expression of the thermostable direct hemolysin gene in Vibrio parahaemolyticus

Bacteria and plasmids

Commentsa

References

V. parahaemolyticus

  

   RIMD2210633

tdh+ (serotype O3:K6; clinical isolate)

19

   Δhfq

hfq mutant derived from RIMD2210633

This study

   pHfq/Δhfq

Δhfq harboring pHfq

This study

   pSN1/Δhfq

Δhfq harboring pSN1

This study

   pHN1/WT

RIMD2210633 harboring pHN1

This study

   pHN2/WT

RIMD2210633 harboring pHN2

This study

   pHRP309/WT

RIMD2210633 harboring pHRP309

This study

   pHN1/Δhfq

Δhfq harboring pHN1

This study

   pHN2/Δhfq

Δhfq harboring pHN2

This study

   pHRP309/Δhfq

Δhfq harboring pRP309

This study

E. coli

  

   SM10λpir

R6Kori λpir supE thi thr leu lacY supE

39

Plasmids

  

   pYK1

Suicide vector; Cmr

39

   pSA19CP-MCS

For gene complementation; Cmr

40

   pHRP309

For β-galactosidase assay; lacZ gen

47

   pSN1

pSA19CP-MCS containing PCR-generated tdhA promoter

23

   pHfq

pSN1 containing PCR-generated hfq

This study

   pHN1

pHRP309 containing PCR-generated tdhA promoter

This study

   pHN2

pHRP309 containing PCR-generated tdhS promoter

This study

  1. aCmr, chloramphenicol resistance