From: Hfq regulates the expression of the thermostable direct hemolysin gene in Vibrio parahaemolyticus
Bacteria and plasmids | Commentsa | References |
---|---|---|
V. parahaemolyticus | Â | Â |
   RIMD2210633 | tdh+ (serotype O3:K6; clinical isolate) | 19 |
   Δhfq | hfq mutant derived from RIMD2210633 | This study |
   pHfq/Δhfq | Δhfq harboring pHfq | This study |
   pSN1/Δhfq | Δhfq harboring pSN1 | This study |
   pHN1/WT | RIMD2210633 harboring pHN1 | This study |
   pHN2/WT | RIMD2210633 harboring pHN2 | This study |
   pHRP309/WT | RIMD2210633 harboring pHRP309 | This study |
   pHN1/Δhfq | Δhfq harboring pHN1 | This study |
   pHN2/Δhfq | Δhfq harboring pHN2 | This study |
   pHRP309/Δhfq | Δhfq harboring pRP309 | This study |
E. coli | Â | Â |
   SM10λpir | R6Kori λpir supE thi thr leu lacY supE | 39 |
Plasmids | Â | Â |
   pYK1 | Suicide vector; Cmr | 39 |
   pSA19CP-MCS | For gene complementation; Cmr | 40 |
   pHRP309 | For β-galactosidase assay; lacZ gen | 47 |
   pSN1 | pSA19CP-MCS containing PCR-generated tdhA promoter | 23 |
   pHfq | pSN1 containing PCR-generated hfq | This study |
   pHN1 | pHRP309 containing PCR-generated tdhA promoter | This study |
   pHN2 | pHRP309 containing PCR-generated tdhS promoter | This study |