Figure 3
From: Hfq regulates the expression of the thermostable direct hemolysin gene in Vibrio parahaemolyticus
Hemolytic activity in culture supernatants of parental and derivative strains. Bacteria were cultured in 10 ml of LB medium supplemented with 3% NaCl at 37°C. One hemolytic unit (HU) was defined as in a previous report [45, 46]. The results are expressed as means ± SDs of 5 independent experiments. *, p < 0.05 versus LB; #, p < 0.01 versus WT. LB, negative control; WT, V. parahaemolyticus strain RIMD2210633 (parent strain); Δhfq, hfq deletion strain; pHfq/Δhfq, hfq-complemented deletion strain; pSN1/Δhfq, deletion strain with empty vector.