Table 3 PCR primers used for the identification and characterization of attaching and effacing Escherichia coli strains

astA

East11a

CCA TCA ACA CAG TAT ATC CGA

27

East11b

GGT CGC GAG TGA CGG CTT TGT

bfpA

EP1

AAT GGT GCT TGC GCT TGC TGC

5

EP2

GCC GCT TTA TCC AAC CTG GTA

eae

SK1

CCC GAA TTC GGC ACA AGC ATA AGC

22

SK2

CCC GGA TCC GTC TCG CCA GTA TTC G

eae- α

SK1-LP2

CCC GAA TTC TTA TTT TAC ACA AGT GGC

25

eae- γ

SK1-LP3

CCC GAA TTC TTC TTT TAC ACA AAC CGC

25

eae- β

SK1-LP4

CCC GTG ATA CCA GTA CCA ATT ACG GTC

22

eae- ε

SK1-LP5

AGC TCA CTC GTA GAT GAC GGC AAG CG

22

eae- ζ

SK1-LP6B

TAG TTG TAC TCC CCT TAT CCC

25

eae- ι

SK1-LP7

TTT ATC CTG CTC CGT TTG CT

25

eae-η

SK1-LP8

TAG ATG ACG GTA GAC

25

eae-κ

SK1-LP10

GGC ATT GTT ATC TGT TGT CT

25

eae-θ

SK1-LP11B

GTT GAT AAC TCC TGA TAT TTT A

25

EAF

EAF1

CAG GGT AAA AGA AAG ATG ATA A

27

EAF25

TAT GGG GAC CAT GTA TTA TCA

fliC

FliC up

CAA GTC ATT ATT AC(AC) AAC AGC C

5

FliC down

GAC AT(AG) TT(AG)GA(AGC) ACT TC(GC) GT

stx

VT1

ATT GAG CAA AAT AAT TTA TAT GTG

27

VT2

TGA TGA TGG CAA TTC AGT AT

tir

tir-R

TAA AAG TTC AGA TCT TGA CAT

26

tir Y-P

tirY474-F

CAT ATT TAT GAT GAG GTC GCT C

tir S

tisS478-F

TCT GTT CAG AAT ATG GGG AAT A