Skip to main content

Table 1 Oci A-domains binding pockets and peptide profiles

From: Recombination and selectional forces in cyanopeptolin NRPS operons from highly similar, but geographically remote Planktothrix strains

 

Binding pockets

OciA-A1

OciA-A2

OciB-A3

OciB-A4

OciB-A5

OciB-A6

OciC-A7

 

NIVA CYA 116

DLGFTGAVCK

DFWNIGMVHK

DAQSMGAIIK

DVENAGVVTK

DAFFLGVTFK

DAWTIAGVCK

DAFFLGVTFK

 

NIES 205

DLGFTGAVCK

DFWNIGMVHK

DAEGMGAIIK

DVENAGVVTK

DAFFLGVTFK

DAWTIAGVCK

DAFFLGVTFK

NIVA CYA 116

        

Mass Da

Side chain

AA 1

AA2

AA3

AA4

AA5

AA6

AA7

1138

HO3-SO-CH2-CH(OMe)-COH

HTyr

Thr

HTyr

Ahp

Ile

Phe(Me)

Ile

1124

HO3-SO-CH2-CH(OMe)-COH

HTyr

Thr

HTyr

Ahp

Val

Phe(Me)

Ile

1124

HO3-SO-CH2-CH(OMe)-COH

HTyr

Thr

HTyr

Ahp

Ile

Phe(Me)

Val

1074

HO3-SO-CH2-CH(OMe)-COH

HTyr

Thr

Ile/Leu

Ahp

Ile

Phe(Me)

Ile

1010

HO3-SO-CH2-CH(OMe)-COH

Ile/Leu

Thr

Ile/Leu

Ahp

Ile

Phe(Me)

Ile

1088

HO3-SO-CH2-CH(OMe)-COH

HTyr

Thr

X

Ahp

Ile

Phe(Me)

Ile

1122

HO3-SO-CH2-CH(OMe)-COH

HTyr

Thr

Y

Ahp

Ile

Phe(Me)

Ile

NIES 205

        

Mass Da

Side chain

AA 1

AA2

AA3

AA4

AA5

AA6

AA7

1138*

HO3-SO-CH2-CH(OMe)-COH

Htyr

Thr

Htyr

Ahp

Ile

Phe(Me)

Ile

1074

HO3-SO-CH2-CH(OMe)-COH

Htyr

Thr

Ile/Leu

Ahp

Ile

Phe(Me)

Ile

1128**

HO3-SO-CH2-CH(OMe)-COH

Htyr

Thr

HcAla

Ahp

Ile

Phe(Me)

Ile

  1. The binding pocket residues of the NIVA CYA 116 and NIES 205 A-domains were identified by comparison to the GrsA-Phe A-domain (Residue 235, 236, 239, 278, 299, 301, 322, 330, 331, 517). The Oci-A3 binding pockets are different (in grey) between the two strains, and the divergent amino acids are shown in bold. The composition of cyanopeptolins produced by NIVA CYA 116 and NIES 205 and their molecular weights (M+H+) are shown with amino acids correlated with the putative binding pockets. HcAla is 3-(4'-hydroxy-2'-cyclohexenyl) alanine, and X and Y is unidentified amino acid derivates. Mutual peptides in NIVA CYA 116 and NIES 205 are highlighted in dark grey and light grey. See [Additional file 1 figure 1, 2 and 3 for the peptide structure and more details on MS data.* peptide named oscillapeptin E and **peptide named oscillapeptin D