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Table 4 Differential amplification kinetics for the SNP alleles.

From: Assignment of Streptococcus agalactiae isolates to clonal complexes using a small set of single nucleotide polymorphisms

SNP

ΔCT ± SD (allele 1)

ΔCT ± SD (allele 2)

ΔΔCT

glnA36

7.6 ± 1.0 (C)

7.5 ± 1.2 (T)

15.1

glnA429

10.9 ± 3.0 (C)

3.9 ± 2.5 (T)

14.8

glcK180

8.5 ± 2.4 (A)

11.1 ± 1.7 (G)

19.6

adhP111

14.9 ± 1.6 (G)

9.5 ± 2.0 (A)

24.4

Atr351 (safety net)

2.1 ± 0.6 (T)

6.6 ± 0.5 (A)

8.7

  1. The ΔCT is the CT for the reaction containing the mis-matched primer – the CT for the reaction containing the matched primer. SD is the standard deviation. The ΔΔCT is the difference between the two ΔCT values and represents the SNP signal.
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BMC Microbiology

ISSN: 1471-2180

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