Evolutional selection of a combinatorial phage library displaying randomly-rearranged various single domains of immunoglobulin (Ig)-binding proteins (IBPs) with four kinds of Ig molecules

Table 1 Sequence analyses of inserted fragments on phage clones in the original library and the third or fourth post-selection libraries with four Ig molecules

Phage Libraries	Composition of single domains of inserted fragment
The original phage library (5*)	PG-PL_9N; PA(A)-PG-PL_6N; PG_3N^R-PG^R-PA(D)_6N; PL_3N-PA(D); PG_6N-PA(A)_3N-PL^R
The 4th round of selection with hIgG (10)	PA(A)-PG_9N(5**)_;PA(A)-PG(2); PA(A)-PG_3N(2); PA(A)_6N-PG_3N
The 4th round of selection with hIgG1-Fc (10)	PA(A)-PG(7); PA(A)_6N-PG(2); PA(A)_3N-PG-PA(A)
The third round of selection with hIgM (11)	PA(A)-PL-PL_9N(4); PA(A)_6N-PL_9N(2); PA(A)-PL_3N(3); PA(A)_9N-PL_3N; PA(A)_9N-PL
The 4th round of selection with hIgA (10)	PA(A)-PL-PL_9N(5); PA(A)_6N-PL_9N(2); PA(A)-PL_3N(2); PA(A)_9N-PL_3N

*: Number of sequenced phage clones; **: number of phage clones with the same inserted fragment; PA(A) and PA(D): A and D domains of protein A; PG: B2 domain of protein G; PL: B3 domain of protein L; 3N, 6N and 9N: the sequence of random linking peptides composed of three, six and nine nucleotides; R: reverse sequence of original sequence.

ISSN: 1471-2180