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Figure 2 | BMC Microbiology

Figure 2

From: CCL5 regulation of mucosal chlamydial immunity and infection

Figure 2

Chlamydia-specific CD4+ T cell proliferation. Groups of naïve or female BALB/c mice challenged with C. muridarum and received 100 μl of control Ab or anti-CCL5 Ab solution every 3 days. Following sacrifice 42 days after challenge, spleen-, ILN-, fallopian tubes-, uterus-, and cervix-derived CD4+ T cells were purified and cultured at a density of 5 × 106 cells/ml with 106 cells/ml of γ-irradiated feeder splenocytes for 3 days. Proliferation of CD4+ T cells was measured by BrdU incorporation. The data presented are the mean OD450 for proliferative ± SEM of quadruplicate cultures. Experiments were repeated 3 times to yield 15 mice per group. Asterisks (*) indicate statistically significant differences (p < 0.01) between untreated, control Ab-treated mice and mice treated with anti-CCL5 Ab.

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