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Table 1 Genes and primers used in this study.

From: Cytokine responses in primary chicken embryo intestinal cells infected with Campylobacter jejuni strains of human and chicken origin and the expression of bacterial virulence-associated genes

Genes (Species)

Primer sequences (5'-3')

Annealing temperatures (°C)

Amplicons (bp)

Standard curves

Correlation coefficient (r2)

GenBank access no.

β-actin (Gallus gallus/chicken)

GAGAAATTGTGCGTGACATCA CCTGAACCTCTCATTGCCA

63

152

y=-3.312x+19.3

0.997

L08165

IL-1β(Gallus gallus/chicken)

CTGGGCATCAAGGGCTACA GGCTGTCCAGGCGGTAGA

62

142

y=-3.359x+25.3

0.990

AJ245728

IL-6 (Gallus gallus/chicken)

GTTCGCCTTTCAGACCTAC ACCACTTCATCGGGATTTA

60

138

y=-3.288x+23.1

0.985

AJ309540

CXCLi1 (K60) (Gallus gallus/chicken)

CAAGCACGTTCAGCGATT ATTCTTGCAGTGAGGTCCG

57

117

y=-3.246x+24.79

0.994

AF277660

CXCLi2 (CAF/IL-8) (Gallus gallus/chicken)

TTGGAAGCCACTTCAGTCAGAC GGAGCAGGAGGAATTACCAGTT

60

120

y=-3.234x+22.5

0.993

AJ009800

TGF-β4 (Gallus gallus/chicken)

AGGATCTGCAGTGGAAGTGGAT CCCCGGGTTGTGTTGGT

61

137

y=-3.145x+23.54

0.990

M31160

iNOS (Gallus gallus/chicken)

CAGCTGATTGGGTGTGGAT TTTCTTTGGCCTACGGGTC

58

158

y=-3.245x+25.8

0.944

U46504

16S (C. jejuni) rRNA (C. jejuni)

AACCTTACCTGGGCTTGATA CTTAACCCAACATCTCACGA

52

122

y=-3.149x+15.7

0.998

NC_002163

ciaB (C. jejuni)

ATATTTGCTAGCAGCGAAGAG GATGTCCCACTTGTAAAGGTG

51

157

y=-3.357x+23.7

0.996

NC_002163

dnaJ (C. jejuni)

AGTGTCGAGCTTAATATCCC GGCGATGATCTTAACATACA

51

117

y=-3.166x+26.3

0.996

NC_002163

racR (C. jejuni)

CTTAAGCGATAAAGTTGTGG CTTTTTGTGCGACGAAT

51

114

y=-3.122x+23.7

0.996

NC_002163