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Figure 3 | BMC Microbiology

Figure 3

From: Transcriptional analysis of Mycobacterium fortuitum cultures upon hydrogen peroxide treatment using the novel standard rrn A-P1

Figure 3

Northern blot analysis of the sod A, kat GII and rrs under hydrogen-peroxide treatment. Total RNA from M. fortuitum cultures treated with H2O2: lanes 1 to 3, 0.02 mM; lanes 4 to 6, 0.2 mM; lanes 7 to 9, 2 mM; lanes 10 to 12, 20 mM and lane C, control. Samples of each concentration were treated using increasing length of periods as follows: 30 min (lanes 1, 4, 7 and 10); 60 min (lanes 2, 5, 8, and 11); 120 min (lanes 3, 6, 9 and 12). The same filter was stripped out and re-hybridized sequentially with the several radiolabelled probes: rrs, kat GII and sod A. The sizes of the corresponding mRNAs are indicated.

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