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Figure 2 | BMC Microbiology

Figure 2

From: Transcriptional analysis of Mycobacterium fortuitum cultures upon hydrogen peroxide treatment using the novel standard rrn A-P1

Figure 2

rrn promoter-usage under hydrogen-peroxide treatment. (a) Percentage of usage corresponding to representative experiment of the M. fortuitum rrn promoters. rrn A-P1 to PCL1 and rrn B-P1 indicate the five promoter of rrn operons in M. fortuitum [48]. (b) Validation of the rrn A-P1 promoter as qRT-PCR reference product; by definition, the value of the control is one (see Methods), the statistical significance of data was determined by applying one-way analysis of variance. M. fortuitum cultures treated with H2O2 at the following concentrations: lanes 1 to 3, 0.02 mM; lanes 4 to 6, 0.2 mM; lanes 7 to 9, 2 mM; lanes 10 to 12, 20 mM and lane C, control. Samples of each concentration were treated using increasing length of periods as follows: 30 min (lanes 1, 4, 7 and 10); 60 min (lanes 2, 5, 8 and 11); and 120 min (lanes 3, 6, 9 and 12). Abbreviations: Ct: Cycle threshold.

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