Figure 1
Protein activity of KatG and SodA using ND-PAGE gels. (a) Catalase and peroxidase activities; (b) Superoxide dismutase activity. M. fortuitum cultures treated with H2O2 at the following concentrations: Lanes 1 to 3, 0.02 mM; lanes 4 to 6, 2 mM; lanes 7 to 9, 20 mM and lane C, control. Samples of each concentration were treated using increasing length of periods as follows: 30 min (lanes 1, 4, and 7); 60 min (lanes 2, 5, and 8); and 120 min (lanes 3, 6, and 9). The bands corresponding to KatGI and KatGII are indicted with arrows. E. coli extracts are incorporated in the catalase-peroxidase activity gel and commercial Fe- and Mn-SOD in the superoxide dismutases activity gel. Abbreviations: Ec: E. coli, Mn: Manganese-SOD, Fe: Iron-SOD.