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Figure 4 | BMC Microbiology

Figure 4

From: Ecological study of Paracoccidioides brasiliensis in soil: growth ability, conidia production and molecular detection

Figure 4

Nested PCR from soil samples with inner primers PbITSE and PbITSR. Lanes 1 and 16: 100 bp DNA ladder; Lane 13: soil seeded with P. brasiliensis (soil positive control); Lanes 14 and 27: DNA positive control; Lanes 15 and 28: negative control; Lanes 5, 7, 8, 9, 11, 12, 20, 21, 22, 23 and 26: positive soil samples (clayey and sandy) collected from armadillo burrows. Lanes 2, 3, 4 and 19: negative soil samples collected from surface; Lanes 6, 10, 17, 18, 24 and 25: negative soil samples collected from armadillo burrows.

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