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Figure 1 | BMC Microbiology

Figure 1

From: IS1111 insertion sequences of Coxiella burnetii: characterization and use for repetitive element PCR-based differentiation of Coxiella burnetii isolates

Figure 1

Proposed algorithm flowchart and agarose gel photographs of corresponding PCR products. To discriminate isolates of C. burnetii using the algorithm shown, PCR is performed using the primer pairs depicted in boxes and negative PCR reactions allow for the discrimination of isolates into the genomic groups shown. Agarose gels of PCR products using primer pairs (from top to bottom) IS 9 and IS1111-1, IS 20 and IS1111-1, IS 5 and IS1111-1, and IS 14 and IS1111-1. Lanes 1, 100 bp ladder; 2, negative control; 3, 9Mi/I; 4, 9Mi/II; 5, RSA 514; 6, 9Mi/Baca; 7, Scottish; 8, Ohio; 9, Australian QD; 10, Q195; 11, Henz I; 12, Henz II; 13, M44; 14, KAV Q154; 15, PAV Q173; 16, Q238; 17, WAV.

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BMC Microbiology

ISSN: 1471-2180

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