Figure 3From: The Yersinia enterocolitica type three secretion chaperone SycO is integrated into the Yop regulatory network and binds to the Yop secretion protein YscM1Overproduction of SecB does not cause significant effects on secretion and expression of Yops. Analogous to the experimental setup described in legend to Fig. 2, SecB was overproduced. (A) Coomassie-stained SDS-PAGE after loading of TCA-precipitated fractions of the culture supernatant. (B) Analysis of the corresponding cell lysates; Coomassie-stained gel (upper panel) and corresponding immunoblots to detect SecB, YopH, and YopE (lower panels).Back to article page