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Table 3 Regulation of sifA by ssrAB.

From: Systematic analysis of the regulation of type three secreted effectors in Salmonella enterica serovar Typhimurium

 

LB

MgM

WT sifA-lac

5.5 ± 1.6

26.3 ± 4.8

MM sifA-lac

3.6 ± 0.5

16.8 ± 2.9

MM sifA-lac + p-ssrB

4.8 ± 0.8

29.6 ± 6.9

MM sifA-lac + pWSK29

3.8 ± 0.5

15 ± 5.6

Δ ssrA sifA-lac

3.8 ± 1.1

14.4 ± 2.7

Δ ssrB sifA-lac

3.9 ± 1.4

14.2 ± 2.5

Δ ssrAB sifA-lac

2.7 ± 2.4

15.6 ± 2.6

  1. The β-galactosidase activities of strains harbouring a sifA-lac fusion were measured using ONPG. Each value represents the mean Miller units of at least three experiments ± standard deviation. WT, wild type 14028; MM, multi-mutant [Δ(avrA-invH)1 ssrB::cat sirA4::hyg].
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BMC Microbiology

ISSN: 1471-2180

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