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Table 2 Cross-reactivity of epiA and epiB polypeptides with affinity-purified anti-epiA antibodies PaP

From: Identification of a human immunodominant B-cell epitope within the immunoglobulin A1 protease of Streptococcus pneumoniae

Antibody source

IgG Rec-ELISA (OD) PbP

 

GST-epiA

GST-epiB

GST-SP4

GST

Sera mixture

2.802

1.332

2.866

0.256

Anti-epiA (150 ng mL-1)

2.107

2.124

2.274

0.092

Anti-epiA (30 ng mL-1)

0.601

0.609

0.660

0.047

  1. Pa PRecombinant antigens were PPanalyzed by IgG Rec-ELISA with a homogeneos mixture of human sera (diluted 1:50) or with affinity-purified anti-epiA antibodies
  2. Pb PThe cutoff values for the IgG Rec-ELISA with the recombinant GST-epiA, GST-epiB, and GST-SP4 proteins were calculated as two times the OD readings obtained with the GST. Boldface type, OD values greater than the cutoff values.