Phylogenetic analysis of Shiga toxin 1 and Shiga toxin 2 genes associated with disease outbreaks

BMC Microbiology

Table 3 Primer sequences

Primer Name	Nucleotide Sequence 5'-3'	Target	PCR Conditions^a
	PCR Primers		Denature	Anneal	Extension
BGR1U	TCAACGAAAAATAACTTCGCTGAATCCC	Stx1 Base 1–28	95°C,	61°C,	72°C,
BGR1D	CAGTTAATGTGGTTGCGAAGGAATTTACC	Stx1 Base (1150–1178)	60 sec	60 sec	240 sec
BGR2U	ATGAAGTGTATATTATTTAAATGGGTACTGTG	Stx2 Base 1–32	95°C,	61°C,	72°C,
BGR2D	TCAGTCATTATTAAACTGCACTTCAG	Stx2 Base (1201–1226)	60 sec	60 sec	240 sec
	Sequencing Primers	Target
Stx1SeqF	TGTAACCGCTGTTGTACCTGG	Stx1 Base (367–387)
Stx1SeqR	TTAATACTGAATTGTCATCATCATGC	Stx1 Base 778–803
Stx2SeqF	TTGCATTAGCTTCTGTTAATGCA	Stx2 Base 1000–1022
Stx2SeqR	CATTCCGGAACGTTCCAG	Stx2 Base (433–450)

^a PCR was performed for 30 cycles followed by a final extension step of 10 min at 72°C.

ISSN: 1471-2180