Figure 1
From: DprA/Smf protein localizes at the DNA uptake machinery in competent Bacillus subtilis cells
Fluorescence microscopy of B. subtilis cells grown to competence. A) Wild type cells expressing GFP-RecA, B) Δsmf (smf:: pMutin-smf) cells expressing GFP-RecA, C) Wild type cells expressing GFP-RecA 30 min after addition of chromosomal DNA, D) Δsmf (smf:: pMutin-Smf) cells expressing GFP-RecA 30 min after addition of chromosomal DNA, E) Polar localization of ComGA-CFP in Δsmf cells. Lower panel shows acquisition of the outlines of cells in Nomarski DIC. White arrowhead indicate the polar position of ComGA-CFP in Fig 2E and GFP-RecA in Fig 2B & C; Grey arrow heads indicate the thread like pattern of GFP-RecA after addition of DNA; All images were equally scaled; grey bars 2 μm.