Figure 1
From: Human inflammatory bowel disease does not associate with Lawsonia intracellularis infection
Detection of Lawsonia intracellularis DNA in intestinal specimens by PCR using different sets of primers. Bacterial DNA was prepared from intestinal specimens obtained from organ donors and IBD patients as described in Material and Methods, and PCR-amplified using primers recognizing lyase-like gene (LLG, A), 16S ribosomal subunit (set II, B and set I, D), and Lawsonia surface antigen (LsaA, C). The sample numeration is as described in Table 1. (31, 32) depict porcine fecal DNA samples used as positive controls; (*) – samples used for sequencing.