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Table 3 The Y. pestis gcvA gene encodes an activator protein. Cells were grown in LB to an OD600 of ~0.5 and assayed for β-galactosidase activity [16]. Activity is expressed in Miller units.

From: The Yersinia pestis gcvB gene encodes two small regulatory RNA molecules

Lysogen

Relevant genotype

β-Galactosidase activity

GS1132λgcvB::lacZ

Δ(gcvA gcvB)

<1

GS1132λgcvB::lacZ[pACYC177]

Δ(gcvA gcvB)

<1

GS1132λgcvB::lacZ[pGS335]

Δ(gcvA gcvB)/gcvAEc

399 ± 22

GS1132λgcvB::lacZ[pgcvAYp-p177]

Δ(gcvA gcvB)/gcvAYp

254 ± 21