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Table 2 Regulation of the Y. pestis gcvB+53::lacZ transcriptional fusion in E. coli. Cells were grown in GM media with the indicated supplements to an OD600 of ~0.5 and assayed for β-galactosidase activity [16]. Activity is expressed in Miller units.

From: The Yersinia pestis gcvB gene encodes two small regulatory RNA molecules

Lysogen

Relevant genotype

β-galactosidase activity for cells grown in:

  

GM

GM + glycine

GM + inosine

GS162λgcvBYp+53::lacZ

WT

15 ± 2

173 ± 2

6 ± 3

GS1118λgcvBYp+53::lacZ

ΔgcvA

2 ± 1

3 ± 1

2 ± 1

GS1053λgcvBYp+53::lacZ

gcvR

620 ± 58

419 ± 13

440 ± 174

GS1144λgcvBYp+53::lacZ

ΔgcvB

10 ± 2

140 ± 24

6 ± 2